Annexin A5 is a cellular protein in the annexin group. In flow cytometry, annexin V is commonly used to detect apoptotic cells by its ability to bind to phosphatidylserine, a marker of apoptosis when it is on the outer leaflet of the plasma membrane. The function of the protein is unknown; however, annexin A5 has been proposed to play a role in the inhibition of blood coagulation by competing for phosphatidylserine binding sites with prothrombin and also to inhibit the activity of phospholipase

FITC-labelled recombinant human annexin V shows bright green fluorescence (Ex(max): 488nm, Em(max): 530nm). Typical flow cytometry results. The Annexin V-FITC apoptosis detection kit allows the detection and discrimination between apoptotic and necrotic cells.

Using fluorescently labeled Annexin V the externalization of PS can easily be detected. Annexin V may also bind to PS on late apoptotic and necrotic cells but as the membrane integrity on these cells has been lost, these cells can be distinguished from early apoptotic cells by the use of the impermeant dye propidium iodide. In early-stage apoptosis, the plasma membrane excludes viability dyes such as propidium iodide (PI), 7-AAD, or Fixable Viability Dyes such as eFluor™ 660 or eFluor™ 780. These cells will stain with Annexin V but not a viability dye, thus distinguishing cells in early apoptosis.

Annexin V Apoptosis Assay. Detection of Apoptosis using Annexin V and NC-3000™. Translocation of phosphatidylserine (PS) from the inner to the outer membrane layer happens early in apoptosis and can be detected with Annexin V coupled to a fluorochrome. Request a Quote. Annexin V-FITC staining of apoptosis in Jurkat T cells. Annexin V-FITC staining of Annexin V (or Annexin A5) is a member of the annexin family of intracellular proteins that Annexin V may be conjugated to fluorochromes including Phycoerythrin (PE). This format retains its high affinity for PS and thus serves as a sensitive probe for flow cytometric analysis of cells that are undergoing apoptosis.

Annexin V protein demonstrates high affinity, specificity, and sensitivity for PS and can be used as a marker of early apoptosis. In order to rule out "leaky" necrotic cells, annexin V must be used in tandem with reagents that determine the integrity of the cell membrane, such as propidium iodide (2).

Annexin V-FITC Apoptosis Detection Kit (Catalog #: K101-25, -100, -400; Store at 4oC; Stable for one year) I. Introduction: Annexin V Apoptosis Detection Kit is based on the observation that soon after initiating apoptosis, cells translocate the membrane phosphatidylserine (PS) from the inner face of the plasma membrane to the cell surface. ApopNexin Annexin V FITC Apoptosis Kit The ApopNexin FITC Apoptosis Detection Kit for Flow Cytometry is designed to detect a specific biochemical change in FITC Annexin V staining precedes the loss of membrane integrity which accompanies the latest stages of cell death resulting from either apoptotic or necrotic Development of the Annexin V Apoptosis Assay: • 1990 Andree at al. found that a protein, Vascular Anticoagulant α, bound to phospholipid bilayers in a calcium 3 Mar 2019 The Annexin V Apoptosis Detection Kit utilizes FITC-conjugated Annexin V protein for detecting cells undergoing apoptosis. Sufficient for 100 Detecting apoptosis in living cells by flow cytometry or fluorescence microscopy.

JA1000. RealTime-Glo(TM) Annexin V Apoptosis Assay, 100. Assay Handelsnamn: Annexin V NanoBiT® Substrate, 1,000X. Artikelnummer:

A. Incubation of cells with annexin V-FITC. Induce apoptosis by the desired method. Collect 1–5 x … Annexin V binding assay as a tool to measure apoptosis in differentiated neuronal cells. We describe a rapid and reliable method to quantitate the extent of apoptosis in neuronal cell cultures. 17 rows Annexin V, a 36-kDa calcium-binding protein, binds to PS; therefore, fluorescently labeled Annexin V can be used to detect PS that is exposed on the outside of apoptotic cells.

3.2.3.4 Embryonic stem cells of mice (Participant 4). 143. RF-EMF exposure may influence the bcl-2 mediated apoptotic Rat and human: discrimination of infection versus tumor. glycolysis (FDG), angiogenesis (VEGF), apoptosis (annexin V), proliferation (FLT) or Som ett annat mått på apoptos utförde vi annexin V-analys.
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Model of plasma membrane; Apoptosis induction by UV-irradiation FITC-labelled recombinant human annexin V shows bright green fluorescence (Ex(max): 488nm, Em(max): 530nm). Typical flow cytometry results.

The kit includes the non-fluorescent compound 6-carboxyfluorescein diacetate (6-CFDA), which enters the cell and is hydrolyzed by the esterases present in living cells to the fluorescent compound 6-carboxyfluorescein, indicating that Elabscience® Annexin V-FITC / PI Cell Apoptosis Detection kit is developed to identify apoptotic and necrotic cells. Annexin V is a member of the annexin family, which binds to phosphatidylserine (PS) in a calcium-dependent manner. Annexin V-FITC, the FITC-conjugated format, binds specifically to the PS on the outer leaflet of The Annexin V Apoptosis Detection Kit includes Annexin V (Catalog #100-0329 or Catalog #100-0331 or Catalog #100-0333), Annexin V Binding Buffer (Catalog #100-0334), and 7-AAD (Catalog #75001).
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Annexin V, a 36-kDa calcium-binding protein, binds to PS; therefore, fluorescently labeled Annexin V can be used to detect PS that is exposed on the outside of apoptotic cells. Annexin V can also stain necrotic cells because these cells have ruptured membranes that permit Annexin V to access the entire plasma membrane.

Annexin V may also bind to PS on late apoptotic and necrotic cells but as the membrane integrity on these cells has been lost, these cells can be distinguished from early apoptotic cells by the use of the impermeant dye propidium iodide. Annexin V-FITC Apoptosis Detection Kit | Sigma-Aldrich. Suitable for flow cytometry; Annexin V-FITC kit allows fluorescent detection of annexin V bound to apoptotic cells and quantitative determination by flow cytometry; Detects apoptosis earlier in the process than DNA-based assays such as TUNEL; ADVANCED SEARCH. Why is annexin V staining used to detect apoptosis? Fluorescent conjugates of annexin V are commonly used to identify apoptotic cells. The human vascular anticoagulant annexin V is a 35–36 kDa, Ca 2 +-dependent phospholipid-binding protein that has a high affinity for the anionic phospholipid phosphatidylserine (PS). Annexin V: In normal viable cells, phosphatidylserine (PS) is located on the cytoplasmic surface of the cell membrane.